Invitrogen

IL-4 Monoclonal Antibody (11B11), Alexa Fluor™ 488, eBioscience™

FIGURE: 1 / 40

IL-4 Antibody (53-7041-80) in Flow

Intracellular staining of Th2-polarized mouse splenocytes with Anti-Mouse CD4 APC-eFluor® 780 (Product # 47-0041-82) and 1.0 µg of Rat IgG1 K Isotype Control Alexa Fluor® 488 (Product # 53-4301-80) (left) or 1.0 µg of Anti-Mouse IL-4 Alexa Fluor® 488 (right). Total viable cells, as determined by Fixable Viability Dye eFluor® 450, were used for analysis.

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Product Details

53-7041-80

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 35 publications 35 publications

ELISA (ELISA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

11B11

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469921

Product Specific Information

Description: The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.

Applications Reported: This 11B11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 11B11 antibody has been tested by intracellular staining and flow cytometric analysis of Th2 polarized and stimulated mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Subconjunctival injection of human umbilical cord mesenchymal stem cells alleviates experimental allergic conjunctivitis via regulating T cell response.

Stem cell research & therapy

Li D,Ou Q,Shen Q,Lu MM,Xu JY,Jin C,Gao F,Wang J,Zhang J,Zhang J,Li J,Lu L,Xu GT,Tian H

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Mon Oct 02 00:00:00 EDT 2023

Respiratory mucosal vaccination of peptide-poloxamine-DNA nanoparticles provides complete protection against lethal SARS-CoV-2 challenge.

Biomaterials

Sun S,Li E,Zhao G,Tang J,Zuo Q,Cai L,Xu C,Sui C,Ou Y,Liu C,Li H,Ding Y,Li C,Lu D,Zhang W,Luo P,Cheng P,Gao Y,Tu C,Pitard B,Rosenecker J,Wang B,Liu Y,Zou Q,Guan S

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2023

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

British journal of pharmacology

Liao W,Foo HYC,Tran TNQ,Chai CLL,Wong WSF

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

Dendritic cell Piezo1 directs the differentiation of T_H1 and T_reg cells in cancer.

eLife

Wang Y,Yang H,Jia A,Wang Y,Yang Q,Dong Y,Hou Y,Cao Y,Dong L,Bi Y,Liu G

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Mon Aug 22 00:00:00 EDT 2022

Romidepsin (FK228) regulates the expression of the immune checkpoint ligand PD-L1 and suppresses cellular immune functions in colon cancer.

Cancer immunology, immunotherapy : CII

Shi Y,Fu Y,Zhang X,Zhao G,Yao Y,Guo Y,Ma G,Bai S,Li H

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Fine particulate matter induces airway inflammation by disturbing the balance between Th1/Th2 and regulation of GATA3 and Runx3 expression in BALB/c mice.

Molecular medicine reports

Pang L,Yu P,Liu X,Fan Y,Shi Y,Zou S

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Irradiated whole-cell vaccine suppresses hepatocellular carcinoma growth in mice via Th9 cells.

Oncology letters

Chen J,Ding Y,Huang F,Lan R,Wang Z,Huang W,Chen R,Wu B,Fu L,Yang Y,Liu J,Hong J,Zhang W,Zhang L

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Features of T-cell subset composition in a D-galactose-induced senescence mouse model.

Experimental animals

Kawata K,Suzuki T,Ozawa K,Sekiguchi M

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Fri Aug 06 00:00:00 EDT 2021

Exosome-mediated mRNA delivery in vivo is safe and can be used to induce SARS-CoV-2 immunity.

The Journal of biological chemistry

Tsai SJ,Atai NA,Cacciottolo M,Nice J,Salehi A,Guo C,Sedgwick A,Kanagavelu S,Gould SJ

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

RSV infection-elicited high MMP-12-producing macrophages exacerbate allergic airway inflammation with neutrophil infiltration.

iScience

Makino A,Shibata T,Nagayasu M,Hosoya I,Nishimura T,Nakano C,Nagata K,Ito T,Takahashi Y,Nakamura S

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Fri Oct 22 00:00:00 EDT 2021

Graded RhoA GTPase Expression in Treg Cells Distinguishes Tumor Immunity From Autoimmunity.

Frontiers in immunology

Kalim KW,Yang JQ,Modur V,Nguyen P,Li Y,Zheng Y,Guo F

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Tue Jan 11 00:00:00 EST 2022

Smek1 deficiency exacerbates experimental autoimmune encephalomyelitis by activating proinflammatory microglia and suppressing the IDO1-AhR pathway.

Journal of neuroinflammation

Duan RN,Yang CL,Du T,Liu A,Wang AR,Sun WJ,Li X,Li JX,Yan CZ,Liu QJ

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Mon Jun 28 00:00:00 EDT 2021

SPARC regulation of PMN clearance protects from pristane-induced lupus and rheumatoid arthritis.

iScience

Sangaletti S,Botti L,Gulino A,Lecis D,Bassani B,Portararo P,Milani M,Cancila V,De Cecco L,Dugo M,Tripodo C,Colombo MP

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Fri Jun 25 00:00:00 EDT 2021

Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry.

STAR protocols

Skordos I,Demeyer A,Beyaert R

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Fri Mar 19 00:00:00 EDT 2021

TIRC7 inhibits Th1 cells by upregulating the expression of CTLA‑4 and STAT3 in mice with acute graft‑versus‑host disease.

Oncology reports

Zhu F,Qiu T,Zhu S,Zhao K,Chen C,Qiao J,Pan B,Yan Z,Chen W,Liu Q,Wu Q,Cao J,Sang W,Zeng L,Sun H,Li Z,Xu K

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Diversity in medullary thymic epithelial cells controls the activity and availability of iNKT cells.

Nature communications

Lucas B,White AJ,Cosway EJ,Parnell SM,James KD,Jones ND,Ohigashi I,Takahama Y,Jenkinson WE,Anderson G

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Mon May 04 00:00:00 EDT 2020

High Glucose Intake Exacerbates Autoimmunity through Reactive-Oxygen-Species-Mediated TGF-β Cytokine Activation.

Immunity

Zhang D,Jin W,Wu R,Li J,Park SA,Tu E,Zanvit P,Xu J,Liu O,Cain A,Chen W

53-7041-82 was used in Flow Cytometry to reveal a previously unrecognized mechanism underlying the adverse effects of high glucose intake in the pathogenesis of autoimmunity and inflammation.

Tue Oct 15 00:00:00 EDT 2019

IL-31 plays dual roles in lung inflammation in an OVA-induced murine asthma model.

Biology open

Huang J,Yue H,Jiang T,Gao J,Shi Y,Shi B,Wu X,Gou X

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Tue Jan 15 00:00:00 EST 2019

Keap1-Nrf2 System Plays an Important Role in Invariant Natural Killer T Cell Development and Homeostasis.

Cell reports

Pyaram K,Kumar A,Kim YH,Noel S,Reddy SP,Rabb H,Chang CH

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Tue Apr 16 00:00:00 EDT 2019

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

Heterologous Prime-Boost Combinations Highlight the Crucial Role of Adjuvant in Priming the Immune System.

Frontiers in immunology

Ciabattini A,Pettini E,Fiorino F,Lucchesi S,Pastore G,Brunetti J,Santoro F,Andersen P,Bracci L,Pozzi G,Medaglini D

53-7041 was used in Flow cytometry/Cell sorting to investigate the impact of heterologous prime-boost approaches on the vaccine-specific cellular and humoral responses specific for a mycobacterial vaccine antigen on C57BL/6 mice.

Mon Apr 08 00:00:00 EDT 2019

MicroRNA-126 deficiency enhanced the activation and function of CD4⁺ T cells by elevating IRS-1 pathway.

Clinical and experimental immunology

Chu F,Hu Y,Zhou Y,Guo M,Lu J,Zheng W,Xu H,Zhao J,Xu L

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

Baicalin Downregulates RLRs Signaling Pathway to Control Influenza A Virus Infection and Improve the Prognosis.

Evidence-based complementary and alternative medicine : eCAM

Pang P,Zheng K,Wu S,Xu H,Deng L,Shi Y,Chen X

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Genome-wide analyses reveal the IRE1a-XBP1 pathway promotes T helper cell differentiation by resolving secretory stress and accelerating proliferation.

Genome medicine

Pramanik J,Chen X,Kar G,Henriksson J,Gomes T,Park JE,Natarajan K,Meyer KB,Miao Z,McKenzie ANJ,Mahata B,Teichmann SA

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Wed Oct 24 00:00:00 EDT 2018

Adipose tissue B2 cells promote insulin resistance through leukotriene LTB4/LTB4R1 signaling.

The Journal of clinical investigation

Ying W,Wollam J,Ofrecio JM,Bandyopadhyay G,El Ouarrat D,Lee YS,Oh DY,Li P,Osborn O,Olefsky JM

53-7041 was used in Flow cytometry/Cell sorting to demonstrate the importance of ATB2 cells in obesity-induced insulin resistance and suggest that inhibition of the LTB4/LTB4R1 axis might be a useful approach for developing insulin-sensitising therapeutics.

Wed Mar 01 00:00:00 EST 2017

Critical role of caspase-8-mediated IL-1 signaling in promoting Th2 responses during asthma pathogenesis.

Mucosal immunology

Qi X,Gurung P,Malireddi RK,Karmaus PW,Sharma D,Vogel P,Chi H,Green DR,Kanneganti TD

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Antibiotic-induced perturbations in microbial diversity during post-natal development alters amyloid pathology in an aged APP_SWE/PS1_ΔE9 murine model of Alzheimer's disease.

Scientific reports

Minter MR,Hinterleitner R,Meisel M,Zhang C,Leone V,Zhang X,Oyler-Castrillo P,Zhang X,Musch MW,Shen X,Jabri B,Chang EB,Tanzi RE,Sisodia SS

53-7041 was used in Flow cytometry/Cell sorting to investigate how ABX-induced microbial diversity perturbations during post-natal stages of development coincide with altered host immunity mechanisms and amyloidosis in a murine model of AD.

Tue Sep 05 00:00:00 EDT 2017

Induction of Broad-Based Immunity and Protective Efficacy by Self-amplifying mRNA Vaccines Encoding Influenza Virus Hemagglutinin.

Journal of virology

Brazzoli M,Magini D,Bonci A,Buccato S,Giovani C,Kratzer R,Zurli V,Mangiavacchi S,Casini D,Brito LM,De Gregorio E,Mason PW,Ulmer JB,Geall AJ,Bertholet S

53-7041 was used in Flow cytometry/Cell sorting to describe protective immune responses in mice and ferrets after vaccination with a novel HA-based influenza vaccine.

Fri Jan 01 00:00:00 EST 2016

Anti-Allergic Effect of Oroxylin A from Oroxylum indicum Using in vivo and in vitro Experiments.

Biomolecules & therapeutics

Lee AY,Kang S,Park SJ,Huang J,Im DS

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in ELISA

Sun May 01 00:00:00 EDT 2016

Inadequate activation of the HBsAg-specific Th cells by APCs leads to hyporesponsiveness to HBsAg vaccine in B10.S mice.

Human vaccines & immunotherapeutics

Li X,Xu J,Lv Z,Wang J,Sun S,Zhu W,Wang B,He R,Qu D

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Tue Apr 19 00:00:00 EDT 2016

Triclosan Induces Thymic Stromal Lymphopoietin in Skin Promoting Th2 Allergic Responses.

Toxicological sciences : an official journal of the Society of Toxicology

Marshall NB,Lukomska E,Long CM,Kashon ML,Sharpnack DD,Nayak AP,Anderson KL,Jean Meade B,Anderson SE

53-7041 was used in Flow cytometry/Cell sorting to examine the effects of dermal triclosan exposure on the skin and lymph nodes of mice and in a human skin model to identify mechanisms for augmenting allergic responses.

Tue Sep 01 00:00:00 EDT 2015

Nizatidine, a small molecular compound, enhances killed H5N1 vaccine cell-mediated responses and protects mice from lethal viral challenge.

Human vaccines & immunotherapeutics

Wang S,Wu B,Xue J,Wang M,Chen R,Wang B

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Mon Jan 05 00:00:00 EST 2015

Helminth-induced interleukin-4 abrogates invariant natural killer T cell activation-associated clearance of bacterial infection.

Infection and immunity

Hsieh YJ,Fu CL,Hsieh MH

53-7041 was used in Flow cytometry/Cell sorting to reveal a previously unappreciated role for helminth-induced IL-4 in impairment of iNKT cell-mediated clearance of bacterial coexposure.

Thu May 01 00:00:00 EDT 2014

Prevention or early cure of type 1 diabetes by intranasal administration of gliadin in NOD mice.

PloS one

Funda DP,Fundova P,Hansen AK,Buschard K

53-7041 was used in Flow cytometry/Cell sorting to investigate whether intranasal (i.n.) administration of gliadin or gluten may arrest the diabetogenic process, showing that i.n. administration of gliadin prevents type 1 diabetes in NOD mice.

Tue Jan 13 00:00:00 EST 2015

Genetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice.

Cardiovascular research

Wan W,Lionakis MS,Liu Q,Roffê E,Murphy PM

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

Interleukin-22 as a molecular adjuvant facilitates IL-17-producing CD8+ T cell responses against a HBV DNA vaccine in mice.

Human vaccines & immunotherapeutics

Wu B,Zou Q,Hu Y,Wang B

Published figure using IL-4 monoclonal antibody (Product # 53-7041-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IGG1 induction factor; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402

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Gene Aliases: BSF-1; Il-4; Il4

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UniProt ID: (Mouse) P07750

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Entrez Gene ID: (Mouse) 16189

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Function(s)

cytokine activity cytokine receptor binding interleukin-4 receptor binding growth factor activity interleukin superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-4 Monoclonal Antibody (11B11), Alexa Fluor™ 488, eBioscience™

IL-4 Antibody (53-7041-80) in Flow

Product Details

53-7041-80

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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